Journal: JACC: Basic to Translational Science

Article Title: Cardiopathogenic T Cells Govern Progression and Functional Remodeling in Inflammatory Cardiomyopathy and Chronic Myocarditis

doi: 10.1016/j.jacbts.2025.101458

Figure Lengend Snippet: The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with IgG2b isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .

Article Snippet: Mice were treated with 500 μg of anti-CD4 antibody (clone: YTS191, ECACC, 87072282) or IgG2b isotype control antibody (clone: MPC-11, ECACC, ATCC CCL-167) by intraperitoneal injection twice a week as demonstrated in the respective experiments.

Techniques: Functional Assay, Control, Staining, MANN-WHITNEY

Journal: Cell Reports Medicine

Article Title: Fc-optimized anti-CTLA-4 antibodies increase tumor-associated high endothelial venules and sensitize refractory tumors to PD-1 blockade

doi: 10.1016/j.xcrm.2025.102141

Figure Lengend Snippet: Fc-enhanced mouse anti-CTLA-4 antibodies prune TA-HEVs in the absence of concurrent PD-1 blockade (A) Treatment schedule. i.p., intraperitoneal; control, isotype control antibodies. (B and C) Mean and individual tumor growth. Data were obtained from two independent experiments ( n = 10 mice per group). (D–G) Frequency of FOXP3 + Tregs in tumor, numbers of tumor-infiltrating FOXP3 + Tregs and FOXP3 − Tconv, and ratio of Tconv to Treg cell numbers in tumors following indicated treatments. Each symbol represents an individual mouse ( n = 10, two independent experiments). (H–M) Frequencies of CD45 − CD31 high endothelial cells, TA-HECs, MECA-79 + CD62P + TA-HECs, numbers of TA-ECs and TA-HECs, and mean fluorescence intensity (MFI) of MECA-79 and CD62P in TA-HECs, following indicated treatments, quantified by flow cytometry. Each symbol represents an individual mouse ( n = 10, two independent experiments). (N) Histograms showing expression of PD-L1 in CD45 − CD31 high tumor endothelial cells following indicated treatments, quantified by flow cytometry. MFI of PD-L1 is quantified. Each symbol represents an individual mouse ( n = 4–5). (O) Treatment schedule. i.p., intraperitoneal; control, isotype control antibodies. (P and Q) Mean and individual tumor growth. Data were obtained from two independent experiments (control, n = 8 mice; 9D9-IgG2a + anti-PD-1, n = 10 mice). (R–T) Numbers of tumor-infiltrating FOXP3 + Tregs and FOXP3 − Tconv, and ratio of Tconv to Tregs cell numbers in tumors following indicated treatments. Each symbol represents an individual mouse ( n = 8–10, two independent experiments). (U) Frequency of TA-HECs. Representative dot plots of TA-HECs are shown. Each symbol represents an individual mouse ( n = 8–10, two independent experiments). Data are shown as mean ± SEM. All p values were determined by unpaired two-tailed Student’s t test except for graph in (N) for which p values were determined by one-way ANOVA with Tukey’s multiple comparison test, and for (B) and (P) for which p values were determined by two-way ANOVA. See also .

Article Snippet: InVivo MAb Mouse IgG2b isotype control (clone MPC-11) , Bio X Cell , Cat# BE0086; RRID: AB_1107791.

Techniques: Control, Fluorescence, Flow Cytometry, Expressing, Two Tailed Test, Comparison

Journal: Scientific Reports

Article Title: Performance validation of global MPPT for efficient power extraction through PV system under complex partial shading effects

doi: 10.1038/s41598-025-01816-3

Figure Lengend Snippet: Flowchart of APO-MPC MPPT algorithm.

Article Snippet: This study developed an adapted perturb and observe based model predictive control (APO-MPC) maximum power point tracking (MPPT) approach in MATLAB/Simulink, comprising six series-connected PV modules, a boost converter, and load.

Techniques:

Journal: Scientific Reports

Article Title: Performance validation of global MPPT for efficient power extraction through PV system under complex partial shading effects

doi: 10.1038/s41598-025-01816-3

Figure Lengend Snippet: Schematic diagram of PV system with MPPT controller.

Article Snippet: This study developed an adapted perturb and observe based model predictive control (APO-MPC) maximum power point tracking (MPPT) approach in MATLAB/Simulink, comprising six series-connected PV modules, a boost converter, and load.

Techniques:

Journal: Scientific Reports

Article Title: Performance validation of global MPPT for efficient power extraction through PV system under complex partial shading effects

doi: 10.1038/s41598-025-01816-3

Figure Lengend Snippet: Comparison of various factors for different MPPT algorithms.

Article Snippet: This study developed an adapted perturb and observe based model predictive control (APO-MPC) maximum power point tracking (MPPT) approach in MATLAB/Simulink, comprising six series-connected PV modules, a boost converter, and load.

Techniques: Comparison